DNA Purification by Solid Phase Extraction (SPE) Methods
نویسندگان
چکیده
منابع مشابه
Solid-Phase Extraction (SPE): Principles and Applications in Food Samples.
Solid-Phase Extraction (SPE) is a sample preparation method that is practised on numerous application fields due to its many advantages compared to other traditional methods. SPE was invented as an alternative to liquid/liquid extraction and eliminated multiple disadvantages, such as usage of large amount of solvent, extended operation time/procedure steps, potential sources of error, and high ...
متن کاملRemoval of Triton X-100 from Plasma Samples Using Mixed-Mode Solid Phase Extraction (SPE)
in t ro du C t io n Pharmaceutical dosing vehicle excipients such as Triton X-100 are often added to formulations to facilitate dissolution in dosing media. Unfortunately, these compounds can cause significant matrix effects, typically ion suppression, in LC/MS/MS analyses. The use of fast LC gradients, common in drug discovery, can result in co-elution of target analytes with these compounds. ...
متن کاملSeparation and Purification of Sulforaphane from Broccoli by Solid Phase Extraction
A simple solid-phase extraction (SPE) method for the determination of sulforaphane in broccoli has been developed. The optimal conditions were found to be use of a silica SPE cartridge, and ethyl acetate and dichloromethane as washing and eluting solvents, respectively, which could eliminate interferences originating from the broccoli matrix. The extracts were sufficiently clean to be directly ...
متن کاملFast and Efficient Purification of Synthetic Peptides by Solid-phase Extraction
Purification of peptides is often the most difficult part of the process used to obtain the compounds [1]. Several methods are used for isolation and subsequent purification of the post-synthesis mixture. Depending on the properties of the peptide and the types of impurity, commonly used techniques are ion-exchange chromatography and gel filtration [2,3]. The standard method for isolation is hi...
متن کاملPurification of Derivatized Oligosaccharides by Solid Phase Extraction for Glycomic Analysis
Profiling of glycans released from proteins is very complex and important. To enhance the detection sensitivity, chemical derivatization is required for the analysis of carbohydrates. Due to the interference of excess reagents, a simple and reliable purification method is usually necessary for the derivatized oligosaccharides. Various SPE based methods have been applied for the clean-up process...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Hacettepe Journal of Biology and Chemistry
سال: 2016
ISSN: 1303-5002
DOI: 10.15671/hjbc.20164420568